Serum: Submit a serum separator tube (SST) or red-top tube (no gel). The tube should be centrifuged after clotting and remain unopened. Plasma: Collect in an EDTA (lavender-top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic screw-cap vial, label as plasma, and submit for testing. Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.

1.0 ml Serum 2-8 ?C

Collection Instructions Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25? C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. If shipping frozen, transfer plasma to a leak-proof transport tube, then freeze prior to shipment. Follow manufacturer's instructions for collection tube handling.

2.0 ml EDTA Plasma Frozen

A separate EDTA (lavender-top), ACD (yellow-top) or sodium heparin (green-top) tube must be submitted for all HLA testing. If submitted with any other test requiring an EDTA, ACD or sodium heparin tube, please submit a separate tube for this test. Do not open tube.

10.0 ml EDTA Blood 2-8 ?C

Collection Instructions Received within 48-72 hours of draw is preferred

5.0 ml EDTA Blood - Room Temperature

Received within 48-72 hours of draw is preferred EDTA (lavender-top) tube cannot be used for other testing prior to HLA testing

10.0 ml EDTA Blood 2-8 ?C

EDTA (lavender) tubes cannot be used for other testing prior to HLA testing

5.0 ml EDTA Plasma 2-8 ?C

1.0 ml Serum 2-8 ?C

10.0 ml random urine 2-8 ?C

It is preferable for the patient to be off medications for three days prior to collection. However, common antihypertensives (diuretics, ACE inhibitors, calcium channel blockers, alpha and beta blockers) cause minimal or no interference. Patient should avoid alcohol, coffee, tea, tobacco and strenuous exercise prior to collection.

5.0 ml random urine with 6N HCL

1 ml serum Frozen collected in a red-top tube (no gel)

Frish Blood sample

Collection Instructions Label the tubes according to your standard operating procedure, making sure that each tube has at least two unique patient identifiers.

2.0 ml Serum 2-8 ?C

Collection Instructions Sample collected in a red-top tube must be allowed to clot for at least 30 minutes but no longer than 1 hour

1.0 ml serum frozen

Cervical swab, Urethral swab, conjunctiva swab , urine, tears, sperm 2-8 ?C

Collection Instructions Normal phlebotomy procedure. Specimen stability is crucial. Store and ship room temperature immediately. Do not freeze.

5.0 ml EDTA Blood - Room Temperature

Patient Preparation Collect plasma specimens after an overnight fast (or at least 4 hours after a meal). Non-fasting samples are acceptable for pediatric patients.

2.0 ml Frozen Heparine Plasma

5.0 ml EDTA Blood - Room Temperature

Patient Preparation Overnight fasting is preferred

3.0 ml serum 2-8 ?C

1.0 ml Serum 2-8 ?C

1.0 ml Serum 2-8 ?C

1.0 ml Serum 2-8 ?C

2.0 ml Serum 2-8 ?C

2.0 ml Serum 2-8 ?C

1.0 ml Serum 2-8 ?C