Patient Preparation: For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. Collection Instructions: Collect blood in an EDTA (lavender-top) tube. Separate plasma as soon after collection as possible. Keep refrigerated (cold packs) after separation.

2.0 ml EDTA Plasma 2-8 ?C

Collection Instructions Do not use preservatives. Acidified specimen is not acceptable. Second morning void urine: Discard the first morning void. Collect the second morning void. Mix well.

10.0 ml random urine 2-8 ?C

Fresh stool sample 2-8 ?C

3.0 ml serum 2-8 ?C

Both CSF & serum are required specimens. It is preferred that the collection date and time be the same for both; however, it is acceptable for them to be drawn within 48 hours of each other. If no serum is supplied, the client will be contacted to confirm the order. CSF collection tube #4 is preferred. Submitting 4th lumbar puncture collection tube minimizes blood contamination.

1.0 ml Serum + 1.0 ml CSF 2-8 C

2.0 ml EDTA Plasma 2-8 ?C

20 ml random urine 2-8 ?C without preservative

Collection Instructions Sterile screw-cap container. Freeze immediately. Ship frozen. Do not thaw. Patient age should be included.

10.0 ml random urine 2-8 ?C

Collection Instructions Sterile screw-cap container. Freeze immediately. Ship frozen. Do not thaw. Patient age should be included.

10.0 ml 24hrs urine 2-8 ?C no preservative

Collection Instructions The following information should be provided with the specimen: Date of collection, age of the patient. Urine samples should not have any preservatives added. Freeze as soon as possible after collection. Ship frozen. Do not thaw.

10.0 ml urine FROZEN

Patient's age and sex are required. Specimens must be received at the laboratory within 24 hours of collection Monday through Thursday. Ship overnight express. Do not use PST (Plasma Separation Tube) heparin tube. Do not pop/open tube. Do not pour off sample. Sample must be sterile.

2.0 ml Heparine blood 2-8 ?C

Patient preparation: Dietary supplements containing biotin may interfere in assays and may skew results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. Allow blood to clot at room temperature (20-26? C) and centrifuge immediately to separate the serum from the cells. Freeze as soon as possible.

1.0 ml Serum Frozen - Fasting

Patient Preparation It is recommended that patients refrain from taking excessive amounts of vitamin C or vitamin C rich food for at least 48 hours prior to collection. Collection Instructions Promptly centrifuge with refrigeration and separate serum or plasma into chilled transport tube. Freeze immediately and ship with dry ice. Ascorbic acid at very high concentration (exceeding 51 mcmol/mL plasma) can interfere.

2.0 ml serum Frozen

Patient should refrain from taking excessive amounts of ascorbic acid or oxalate-rich foods (i.e., spinach, coffee, tea, chocolate, rhubarb) for at least 48 hours prior to collection period

24 urine collection

2.0 ml Serum 2-8 ?C ( no gel separator)

1.0 ml Serum 2-8 ?C

Collection Instructions Collect undiluted feces in clean, dry, sterile leak-proof container. Do not add fixative or preservative.

1g. faeces 2-8 ?C

1. Insert collection device into the endocervix until only the bottom-most bristles are exposed at the os. Slowly rotate 1/4-1/2 turn in one direction. To reduce unnecessary bleeding, do not over-rotate device. Remove collection device. 2. Rinse the collection device in the PreservCyt Solution by rotating the device in the solution 10 times while pushing against the vial walls. Swirl the brush vigorously to further release material. Discard the collection device. Do not let the collection device sit in the vial. 3. Tighten the PreservCyt vial cap on the vial. Label PreservCyt vial with patient name and send to lab with cytology requistion in a specimen bag.

PAP Smear

Sure path or Thin Prep

Patient Preparation Collect as a trough just prior to next dose

2.0 ml Serum 2-8 ?C ( no gel separator)

Unstained paraffin section

Unstained paraffin section

Unstained paraffin section

Paraffin Block