Collection Instructions Tumor paraffin block (formalin-fixed only) or five 4-micron unstained sections on Poly-L-Lysine or Silane coated slides. Please submit stained H & E slide and a copy of the pathology report. Ship at room temperature. Do not place paper labels with adhesive backing on slides. Use pencil or xylene resistant pen to write on the frosted end of the slide only.

Paraffin Block

1.0 ml Serum 2-8 ?C

2.0 ml Serum 2-8 ?C

10.0 ml Heparine blood 2-8 ?C

Invert tube 5 to 10 times immediately after filling and transport at room temperature (stable for 24 hrs). NOTE: For patients on heparin, specimen must be centrifuged within 1 hour of collection and tested within 4 hours if kept at room temperature. To increase stability send frozen platelet-poor plasma. For frozen platelet-poor specimens: Centrifuge the specimen for 15 minutes within 1 hour of collection. Using a plastic pipette, remove the plasma taking care to avoid the WBC/platelet buffy layer and transfer the plasma into a properly labeled transfer tube. Centrifuge the transferred plasma a second time and transfer platelet-poor plasma into a new properly labeled transfer tube. Label tube as "platelet-poor". Freeze immediately and transport to the lab frozen. *Send both the transfer tube (frozen) and the original tube (room temp) to the lab.

1.0 ml Citrated plasma ,1: 9 ( Frozen )

2.0 ml Serum 2-8 ?C

Paraffin Block

Collection Instructions CSF: Submit in a sterile, plastic container and store refrigerated or frozen. Note: Avoid repeated freezing and thawing.

2.0 ml EDTA blood, swab, 1 ml csf or 10 ml urine

2.0 ml EDTA blood 2-8 ?C

5.0 ml Peritoneal Fluid 2-8 ?C

5.0 ml Peritoneal Fluid 2-8 ?C

5.0 ml Peritoneal Fluid 2-8 ?C

Patient Preparation Collect just prior to next dose

2.0 ml Serum 2-8 ?C ( no gel separator)

10.0 ml random urine 2-8 ?C

10.0 ml random urine 2-8 ?C

Patient Preparation Collect specimen after an overnight fast (or at least 4 hours after a meal) if possible. Non-fasting samples are acceptable for pediatric patients. Separate plasma from cells as soon as possible and freeze below -20? C

2.0 ml Frozen Heparine Plasma

Patient Preparation Collect as a trough just prior to next dose. For patients receiving fosphenytoin therapy, collect as a trough at least 2 hours after IV infusion or at least 4 hours after IM injection.

1.0 ml Serum 2-8 ?C

Frish Blood sample

2.0 ml Serum 2-8 ?C

1.0 ml Serum 2-8 ?C

1.0 ml Serum 2-8 ?C

1.0 ml Serum 2-8 ?C

Urine should be acidified with 25 ml of 6 N HCL added to the container before collection begins. The collection container must be kept refrigerated or on ice during collection. Instruct patient to void and discard the specimen. Note the time, and then collect all urine passed for the next 24 hours (same time the following day). Refer to separate patient instructions for detailed 24-hour urine collection. Record the collection date/time and total volume on the specimen container and test requisition.

10.0 ml of 24hrs urine ?2-8 ?C

1.0 ml EDTA Plasma 2-8 ?C